Peptide-Carrier Conjugation

Methods Mol Biol. 2015:1348:51-7. doi: 10.1007/978-1-4939-2999-3_6.

Abstract

To produce antibodies against synthetic peptides it is necessary to couple them to a protein carrier. This chapter provides a nonspecialist overview of peptide-carrier conjugation. Furthermore, a protocol for coupling cysteine-containing peptides to bovine serum albumin is outlined.

Keywords: Bovine serum albumin; Peptide-carrier conjugation; m-Maleimidobenzoyl-N-hydroxysuccinimidyl ester (MBS).

MeSH terms

  • Animals
  • Carrier Proteins / chemistry*
  • Carrier Proteins / immunology
  • Cattle
  • Cysteine / chemistry
  • Peptides / chemistry*
  • Peptides / immunology
  • Serum Albumin, Bovine / chemistry
  • Serum Albumin, Bovine / immunology
  • Succinimides / chemistry

Substances

  • Carrier Proteins
  • Peptides
  • Succinimides
  • m-maleimidobenzoyl-N-hydroxysulfosuccinimide ester
  • Serum Albumin, Bovine
  • Cysteine