Characterization of 14 microsatellite markers for Silene acaulis (Caryophyllaceae)(1)

Eike Müller; Iva Hlaváčková; Mildrid Elvik Svoen; Inger Greve Alsos; Pernille Bronken Eidesen

doi:10.3732/apps.1500036

Characterization of 14 microsatellite markers for Silene acaulis (Caryophyllaceae)(1)

Appl Plant Sci. 2015 Sep 8;3(9):apps.1500036. doi: 10.3732/apps.1500036. eCollection 2015 Sep.

Authors

Eike Müller¹, Iva Hlaváčková², Mildrid Elvik Svoen³, Inger Greve Alsos⁴, Pernille Bronken Eidesen¹

Affiliations

¹ University Centre in Svalbard, Postboks 156, NO-9170 Longyearbyen, Norway.
² Institute of Chemical Technology, University of Chemistry and Technology (UCT) Prague, Technická 5, 16628 Prague, Czech Republic.
³ University Centre in Svalbard, Postboks 156, NO-9170 Longyearbyen, Norway ; University of Oslo, Postboks 1072 Blindern, NO-0316 Oslo, Norway.
⁴ University Centre in Svalbard, Postboks 156, NO-9170 Longyearbyen, Norway ; Tromsø Museum, University of Tromsø, Postboks 6050 Langnes, NO-9037 Tromsø, Norway.

Abstract

Premise of the study: Fifty candidate microsatellite markers, generated using 454 shotgun sequencing, were tested for the widespread arctic/alpine herb Silene acaulis (Caryophyllaceae).

Methods and results: Fourteen out of 50 markers resulted in polymorphic products with profiles that enabled interpretation. The numbers of alleles per locus ranged from two to six, and the expected heterozygosity per locus ranged from 0.06 to 0.68. Analysis of F0 and F1 samples proved that one allele was always inherited maternally. Four multiplex mixes have been developed.

Conclusions: Microsatellite markers for this species will be a valuable tool to study detailed small-scale genetic patterns in an arctic/alpine herb and to relate them to demographic parameters.

Keywords: Arctic/alpine; Caryophyllaceae; Silene acaulis; gynodioecious; moss campion.