Metabolomic charactetization of yeast cells after dehydration stress

Int Microbiol. 2014 Sep;17(3):131-9. doi: 10.2436/20.1501.01.215.

Abstract

In this study, we analyzed the metabolite features of the yeasts Saccharomyces cerevisiae, Naumovia castellii, and Saccharomyces mikatae. The three species are closely related genetically but differ in their tolerance of desiccation stress. Specifically, we determined whether certain metabolites correlated with cell viability after stress imposition. The metabolomics profiles of these strains were compared before cell desiccation and after cell rehydration. In S. mikatae, the presence of lysine or glutamine during rehydration led to a 20% increase in survival whereas during dehydration the levels of both amino acids in this yeast were drastically reduced.

Keywords: Naumovia castellii; Saccaromyces mikatae; Saccharomyces cerevisiae; dehydration stress; metabolite extraction; viability; wild yeast.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acids / metabolism
  • Desiccation
  • Microbial Viability
  • Saccharomyces / chemistry
  • Saccharomyces / genetics
  • Saccharomyces / metabolism*
  • Saccharomyces cerevisiae / chemistry
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / growth & development
  • Saccharomyces cerevisiae / metabolism*
  • Stress, Physiological
  • Water / analysis
  • Water / metabolism

Substances

  • Amino Acids
  • Water