Widespread nuclease contamination in commonly used oxygen-scavenging systems

Nat Methods. 2015 Oct;12(10):901-2. doi: 10.1038/nmeth.3588.

Authors

Gayan Senavirathne¹, Jiaquan Liu¹, Miguel A Lopez Jr¹, Jeungphill Hanne¹, Juana Martin-Lopez¹, Jong-Bong Lee^{2

3}, Kristine E Yoder¹, Richard Fishel^{1

4}

Affiliations

¹ Department of Molecular Virology, Immunology and Medical Genetics, The Ohio State University Medical Center, Columbus, Ohio, USA.
² Department of Physics, Pohang University of Science and Technology, Pohang, Korea.
³ School of Interdisciplinary Bioscience and Bioengineering, Pohang, Korea.
⁴ Physics Department, The Ohio State University, Columbus, Ohio, USA.

No abstract available

Publication types

Letter
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Biochemistry / methods*
Carbocyanines / chemistry
Catalase / chemistry
Deoxyribonucleases / metabolism*
Free Radical Scavengers / chemistry*
Oxygen / metabolism
Photobleaching
Protocatechuate-3,4-Dioxygenase / chemistry
Protocatechuate-3,4-Dioxygenase / isolation & purification*

Substances

Carbocyanines
Free Radical Scavengers
cyanine dye 3
Catalase
Protocatechuate-3,4-Dioxygenase
Deoxyribonucleases
Oxygen

Grants and funding