Generation of human iPS cell line ihFib3.2 from dermal fibroblasts

Fernanda Cristina Paccola Mesquita; Tais Hanae Kasai-Brunswick; Fernanda de Mello e Souza Valente Gubert; Tamara Borgonovo; Danúbia Silva-dos-Santos; Dayanada Silva de Araújo; Antonio Carlos Campos-de-Carvalho; Adriana Bastos Carvalho

doi:10.1016/j.scr.2015.09.001

Generation of human iPS cell line ihFib3.2 from dermal fibroblasts

Stem Cell Res. 2015 Nov;15(3):445-8. doi: 10.1016/j.scr.2015.09.001. Epub 2015 Sep 10.

Authors

Fernanda Cristina Paccola Mesquita¹, Tais Hanae Kasai-Brunswick¹, Fernanda de Mello e Souza Valente Gubert², Tamara Borgonovo³, Danúbia Silva-dos-Santos², Dayanada Silva de Araújo², Antonio Carlos Campos-de-Carvalho¹, Adriana Bastos Carvalho²

Affiliations

¹ Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil; Instituto Nacional de Cardiologia, Rio de Janeiro, RJ, Brazil.
² Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
³ Pontifícia Universidade Católica do Paraná, Paraná, PR, Brazil.

PMID: 26413783
DOI: 10.1016/j.scr.2015.09.001

Abstract

The human ihFib3.2 iPS cell line was generated from dermal fibroblasts obtained from a healthy donor. Lentiviral particles were produced with the polycistronic hSTEMCCA vector with Oct4, Sox2, cMyc and Klf4 as reprogramming factors.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line
Cells, Cultured
Fibroblasts / metabolism*
Humans
Induced Pluripotent Stem Cells / metabolism*
Kruppel-Like Factor 4