De novo sequencing and comprehensive analysis of the mutant transcriptome from purple sweet potato (Ipomoea batatas L.)

Peiyong Ma; Xiaofeng Bian; Zhaodong Jia; Xiaoding Guo; Yizhi Xie

doi:10.1016/j.gene.2015.09.056

De novo sequencing and comprehensive analysis of the mutant transcriptome from purple sweet potato (Ipomoea batatas L.)

Gene. 2016 Jan 10;575(2 Pt 3):641-9. doi: 10.1016/j.gene.2015.09.056. Epub 2015 Sep 26.

Authors

Peiyong Ma¹, Xiaofeng Bian¹, Zhaodong Jia¹, Xiaoding Guo¹, Yizhi Xie²

Affiliations

¹ Institute of Food Crops, Provincial Key Laboratory of Agrobiology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, Jiangsu, China.
² Institute of Food Crops, Provincial Key Laboratory of Agrobiology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, Jiangsu, China. Electronic address: xyz@jaas.ac.cn.

PMID: 26410411
DOI: 10.1016/j.gene.2015.09.056

Abstract

Purple sweet potatoes, rich in anthocyanin, have been widely favored in light of increasing awareness of health and food safety. In this study, a mutant of purple sweet potato (white peel and flesh) was used to study anthocyanin metabolism by high-throughput RNA sequencing and comparative analysis of the mutant and wild type transcriptomes. A total of 88,509 unigenes ranging from 200nt to 14,986nt with an average length of 849nt were obtained. Unigenes were assigned to Gene Ontology (GO), Clusters of Orthologous Group (COG) and Kyoto Encyclopedia of Genes and Genomes (KEGG). Functional enrichment using GO and KEGG annotations showed that 3828 of the differently expressed genes probably influenced many important biological and metabolic pathways, including anthocyanin biosynthesis. Most importantly, the structural and transcription factor genes that contribute to anthocyanin biosynthesis were downregulated in the mutant. The unigene dataset that was used to discover the anthocyanin candidate genes can serve as a comprehensive resource for molecular research in sweet potato.

Keywords: Anthocyanin; Gene expression; Mutant; Sweet potato; Transcriptome.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Anthocyanins / biosynthesis*
Databases, Genetic
Gene Expression Profiling / methods
Gene Expression Regulation, Plant
Gene Ontology
High-Throughput Nucleotide Sequencing
Ipomoea batatas / chemistry
Ipomoea batatas / classification
Ipomoea batatas / genetics*
Molecular Sequence Annotation
Mutation*
Plant Proteins / genetics*
Plant Proteins / metabolism
Sequence Analysis, RNA / methods*
Transcription Factors / genetics
Transcription Factors / metabolism

Substances

Anthocyanins
Plant Proteins
Transcription Factors