Dual-color photoacoustic lymph node imaging using nanoformulated naphthalocyanines

Changho Lee; Jeesu Kim; Yumiao Zhang; Mansik Jeon; Chengbo Liu; Liang Song; Jonathan F Lovell; Chulhong Kim

doi:10.1016/j.biomaterials.2015.09.023

Dual-color photoacoustic lymph node imaging using nanoformulated naphthalocyanines

Biomaterials. 2015 Dec:73:142-8. doi: 10.1016/j.biomaterials.2015.09.023. Epub 2015 Sep 16.

Authors

Changho Lee¹, Jeesu Kim¹, Yumiao Zhang², Mansik Jeon³, Chengbo Liu⁴, Liang Song⁴, Jonathan F Lovell⁵, Chulhong Kim⁶

Affiliations

¹ Future IT Innovation Laboratory, Department of Creative IT Engineering and Electrical Engineering, Pohang University of Science and Technology (POSTECH), 77 Cheongam-ro, Nam-gu, Pohang, Gyeongbuk, Republic of Korea.
² Department of Biomedical Engineering, University at Buffalo, The State University of New York, Buffalo, NY 14260, USA.
³ Future IT Innovation Laboratory, Department of Creative IT Engineering and Electrical Engineering, Pohang University of Science and Technology (POSTECH), 77 Cheongam-ro, Nam-gu, Pohang, Gyeongbuk, Republic of Korea; School of Electronics Engineering, College of IT Engineering, Kyungpook National University, 80 Daehak-ro, Bukgu, Daegu 702101, Republic of Korea.
⁴ Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, 1068 Xueyuan Boulevard, Shenzhen 518055, China.
⁵ Department of Biomedical Engineering, University at Buffalo, The State University of New York, Buffalo, NY 14260, USA. Electronic address: jflovell@buffalo.edu.
⁶ Future IT Innovation Laboratory, Department of Creative IT Engineering and Electrical Engineering, Pohang University of Science and Technology (POSTECH), 77 Cheongam-ro, Nam-gu, Pohang, Gyeongbuk, Republic of Korea. Electronic address: chulhong@postech.edu.

PMID: 26408999
DOI: 10.1016/j.biomaterials.2015.09.023

Abstract

Demarking lymph node networks is important for cancer staging in clinical practice. Here, we demonstrate in vivo dual-color photoacoustic lymphangiography using all-organic nanoformulated naphthalocyanines (referred to as nanonaps). Nanonap frozen micelles were self-assembled from two different naphthalocyanine dyes with near-infrared absorption at 707 nm or 860 nm. These allowed for noninvasive, nonionizing, high resolution photoacoustic identification of separate lymphatic drainage systems in vivo. With both types of nanonaps, rat lymph nodes buried deeply below an exogenously-placed 10 mm thick layer of chicken breast were clearly visualized in vivo. These results show the potential of multispectral photoacoustic imaging with nanonaps for detailed mapping of lymphatic drainage systems.

Keywords: Naphthalocyanines; Optoacoustic; Organic nanoparticles; Photoacoustic imaging; Sentinel lymph node mapping.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Acoustics
Animals
Breast Neoplasms / diagnosis
Breast Neoplasms / pathology
Carbocyanines / chemistry*
Cell Movement
Chickens
Color
Female
Humans
Lymph Nodes / pathology*
Mice
Mice, Inbred BALB C
Micelles
Nanoparticles / chemistry*
Nanotechnology / methods*
Neoplasm Metastasis
Neoplasm Staging / methods
Optics and Photonics
Rats
Rats, Sprague-Dawley
Sentinel Lymph Node Biopsy
Spectrophotometry / methods

Substances

Carbocyanines
Micelles
cyanine dye 1

Grants and funding

DP5OD017898/OD/NIH HHS/United States