Multiplexed microsatellite markers for seven Metarhizium species

Johanna Mayerhofer; Andy Lutz; Franco Widmer; Stephen A Rehner; Adrian Leuchtmann; Jürg Enkerli

doi:10.1016/j.jip.2015.09.006

Multiplexed microsatellite markers for seven Metarhizium species

J Invertebr Pathol. 2015 Nov:132:132-134. doi: 10.1016/j.jip.2015.09.006. Epub 2015 Sep 25.

Authors

Johanna Mayerhofer¹, Andy Lutz¹, Franco Widmer¹, Stephen A Rehner², Adrian Leuchtmann³, Jürg Enkerli⁴

Affiliations

¹ Molecular Ecology, Institute for Sustainability Sciences, Agroscope, Reckenholzstrasse 191, 8046 Zurich, Switzerland.
² Systematic Mycology and Microbiology Laboratory, USDA-ARS, Beltsville, MD 20705-2350, USA.
³ Plant Ecological Genetics, Institute of Integrative Biology, ETH Zurich, CH-8092 Zurich, Switzerland.
⁴ Molecular Ecology, Institute for Sustainability Sciences, Agroscope, Reckenholzstrasse 191, 8046 Zurich, Switzerland. Electronic address: juerg.enkerli@agroscope.admin.ch.

PMID: 26407949
DOI: 10.1016/j.jip.2015.09.006

Abstract

Cross-species transferability of 41 previously published simple sequence repeat (SSR) markers was assessed for 11 species of the entomopathogenic fungus Metarhizium. A collection of 65 Metarhizium strains including all 54 used in a recent phylogenetic revision of the genus were characterized. Between 15 and 34 polymorphic SSR markers produced scorable PCR amplicons in seven species, including M. anisopliae, M. brunneum, M. guizhouense, M. lepidiotae, M. majus, M. pingshaense, and M. robertsii. To provide genotyping tools for concurrent analysis of these seven species fifteen markers grouped in five multiplex pools were selected based on high allelic diversity and easy scorability of SSR chromatograms.

Keywords: Biocontrol; Cross-species amplification; Molecular genetic identification.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Genetic Variation
Genotyping Techniques
Metarhizium / genetics*
Microsatellite Repeats*
Phylogeny
Sequence Analysis, DNA