The physiological concentration of ferrous iron (II) alters the inhibitory effect of hydrogen peroxide on CD45, LAR and PTP1B phosphatases

Alicja Kuban-Jankowska; Magdalena Gorska; Lukasz Jaremko; Mariusz Jaremko; Jack A Tuszynski; Michal Wozniak

doi:10.1007/s10534-015-9882-4

The physiological concentration of ferrous iron (II) alters the inhibitory effect of hydrogen peroxide on CD45, LAR and PTP1B phosphatases

Biometals. 2015 Dec;28(6):975-86. doi: 10.1007/s10534-015-9882-4. Epub 2015 Sep 25.

Authors

Alicja Kuban-Jankowska¹, Magdalena Gorska², Lukasz Jaremko³, Mariusz Jaremko^{2

4}, Jack A Tuszynski⁵, Michal Wozniak²

Affiliations

¹ Department of Medical Chemistry, Medical University of Gdansk, Gdansk, Poland. alicjakuban@gumed.edu.pl.
² Department of Medical Chemistry, Medical University of Gdansk, Gdansk, Poland.
³ Max Planck Institute for Biophysical Chemistry and German Center for Neurodegenerative Diseases (DZNE), Göttingen, Germany.
⁴ Department of NMR-based Structural Biology, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
⁵ Department of Physics, University of Alberta, Edmonton, Canada.

Abstract

Hydrogen peroxide is an important regulator of protein tyrosine phosphatase activity via reversible oxidation. However, the role of iron in this reaction has not been yet elucidated. Here we compare the influence of hydrogen peroxide and the ferrous iron (reagent for Fenton reaction) on the enzymatic activity of recombinant CD45, LAR, PTP1B phosphatases and cellular CD45 in Jurkat cells. The obtained results show that ferrous iron (II) is potent inhibitor of CD45, LAR and PTP1B, but the inhibitory effect is concentration dependent. We found that the higher concentrations of ferrous iron (II) increase the inactivation of CD45, LAR and PTP1B phosphatase caused by hydrogen peroxide, but the addition of the physiological concentration (500 nM) of ferrous iron (II) has even a slightly preventive effect on the phosphatase activity against hydrogen peroxide.

Keywords: CD45; Ferrous iron; Hydrogen peroxide; LAR; PTP1B.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Dose-Response Relationship, Drug
Drug Interactions
Ferrous Compounds / pharmacology*
Gene Expression Regulation
Humans
Hydrogen Peroxide / pharmacology*
Jurkat Cells
Leukocyte Common Antigens / antagonists & inhibitors
Leukocyte Common Antigens / genetics*
Leukocyte Common Antigens / metabolism
Protein Tyrosine Phosphatase, Non-Receptor Type 1 / antagonists & inhibitors
Protein Tyrosine Phosphatase, Non-Receptor Type 1 / genetics*
Protein Tyrosine Phosphatase, Non-Receptor Type 1 / metabolism
Receptor-Like Protein Tyrosine Phosphatases, Class 2 / antagonists & inhibitors
Receptor-Like Protein Tyrosine Phosphatases, Class 2 / genetics*
Receptor-Like Protein Tyrosine Phosphatases, Class 2 / metabolism
Signal Transduction

Substances

Ferrous Compounds
ferrous sulfate
Hydrogen Peroxide
Leukocyte Common Antigens
PTPN1 protein, human
PTPRC protein, human
Protein Tyrosine Phosphatase, Non-Receptor Type 1
Receptor-Like Protein Tyrosine Phosphatases, Class 2