Development of SPR biosensor for simultaneous detection of multiplex respiratory viruses

Biomed Mater Eng. 2015:26 Suppl 1:S2207-16. doi: 10.3233/BME-151526.

Abstract

A surface plasmon resonance (SPR)-based biosensor was developed for specific detection of nine common respiratory virus, including influenza A and influenza B, H1N1, respiratory syncytial virus (RSV), parainfluenza virus 1-3 (PIV1, 2, 3), adenovirus, and severe acute respiratory syndrome coronavirus (SARS). The SPR biosensor was developed by immobilizing nine respiratory virus-specific oligonucleotides in an SPR chip. To increase the biosensor sensitivity, biotin was used to label the PCR primer and further amplify the signal by introducing streptavidin after hybridization. Throat swab specimens representing nine common respiratory viruses were tested by the innovative SPR-based biosensor to evaluate the sensitivity, specificity and reproducibility of this method. Results suggest that this biosensor has the potential to simultaneously identify common respiratory viruses.

Keywords: SPR; gene chip; high throughput; label-free; respiratory virus.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenoviridae / genetics
  • Adenoviridae / isolation & purification*
  • Adenoviridae Infections / diagnosis
  • Adenoviridae Infections / virology
  • Betainfluenzavirus / genetics
  • Betainfluenzavirus / isolation & purification*
  • Humans
  • Influenza A Virus, H1N1 Subtype / genetics
  • Influenza A Virus, H1N1 Subtype / isolation & purification
  • Influenza A virus / genetics
  • Influenza A virus / isolation & purification*
  • Influenza, Human / diagnosis
  • Influenza, Human / virology
  • Oligonucleotide Array Sequence Analysis
  • Respiratory Syncytial Virus Infections / diagnosis
  • Respiratory Syncytial Virus Infections / virology
  • Respiratory Syncytial Viruses / genetics
  • Respiratory Syncytial Viruses / isolation & purification*
  • Respirovirus / genetics
  • Respirovirus / isolation & purification*
  • Respirovirus Infections / diagnosis
  • Respirovirus Infections / virology
  • Sensitivity and Specificity
  • Severe Acute Respiratory Syndrome / diagnosis
  • Severe Acute Respiratory Syndrome / virology
  • Severe acute respiratory syndrome-related coronavirus / genetics
  • Severe acute respiratory syndrome-related coronavirus / isolation & purification*
  • Surface Plasmon Resonance / methods*