An Apta-Biosensor for Colon Cancer Diagnostics

Sensors (Basel). 2015 Sep 3;15(9):22291-303. doi: 10.3390/s150922291.

Abstract

This paper reports the design and implementation of an aptasensor using a modified KCHA10a aptamer. This aptasensor consists of a functionalized electrodes using various materials including 11-mercaptoandecanoic acid (11-MUA) and modified KCHA10a aptamer. The HCT 116, HT 29 and HEp-2 cell lines are used in this study to demonstrate the functionality of aptasensor for colon cancer detection purposes. Flow cytometry, fluorescence microscopy and electrochemical cyclic voltammetry are used to verify the binding between the target cells and aptamer. The limit of detection (LOD) of this aptasensor is equal to seven cancer cells. Based on the experimental results, the proposed sensor can be employed for point-of-care cancer disease diagnostics.

Keywords: aptamer; aptasensor; biosensor; colon cancer; cyclic voltammetry (CV); fluorescence labeling.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aptamers, Nucleotide / chemistry*
  • Base Sequence
  • Biosensing Techniques / methods*
  • Calibration
  • Cell Line, Tumor
  • Colonic Neoplasms / diagnosis*
  • Computer Simulation
  • Electrochemical Techniques
  • Electrodes
  • Flow Cytometry
  • Humans
  • Microscopy, Fluorescence
  • Molecular Sequence Data

Substances

  • Aptamers, Nucleotide