MicroRNAs in urine are not biomarkers of multiple myeloma

J Negat Results Biomed. 2015 Sep 23:14:16. doi: 10.1186/s12952-015-0035-7.

Abstract

Background: In this study, we aimed to identify microRNA from urine of multiple myeloma patients that could serve as a biomarker for the disease.

Results: Analysis of urine samples was performed using Serum/Plasma Focus PCR MicroRNA Panel (Exiqon) and verified using individual TaqMan miRNA assays for qPCR. We found 20 deregulated microRNA (p < 0.05); for further validation, we chose 8 of them. Nevertheless, only differences in expression levels of miR-22-3p remained close to statistical significance.

Conclusions: Our preliminary results did not confirm urine microRNA as a potential biomarker for multiple myeloma.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Biomarkers, Tumor / urine*
  • Female
  • Gene Expression Profiling / methods
  • Humans
  • Male
  • MicroRNAs / genetics
  • MicroRNAs / urine*
  • Middle Aged
  • Multiple Myeloma / diagnosis*
  • Multiple Myeloma / genetics
  • Multiple Myeloma / urine*

Substances

  • Biomarkers, Tumor
  • MicroRNAs