DNA damage and oxidative stress induced by imidacloprid exposure in the earthworm Eisenia fetida

Juan Wang; Jinhua Wang; Guangchi Wang; Lusheng Zhu; Jun Wang

doi:10.1016/j.chemosphere.2015.09.004

DNA damage and oxidative stress induced by imidacloprid exposure in the earthworm Eisenia fetida

Chemosphere. 2016 Feb:144:510-7. doi: 10.1016/j.chemosphere.2015.09.004. Epub 2015 Sep 19.

Authors

Juan Wang¹, Jinhua Wang², Guangchi Wang¹, Lusheng Zhu¹, Jun Wang¹

Affiliations

¹ National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, Key Laboratory of Agricultural Environment in Universities of Shandong, College of Resources and Environment, Shandong Agricultural University, Taian, 271018, People's Republic of China.
² National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, Key Laboratory of Agricultural Environment in Universities of Shandong, College of Resources and Environment, Shandong Agricultural University, Taian, 271018, People's Republic of China. Electronic address: wjh@sdau.edu.cn.

PMID: 26397468
DOI: 10.1016/j.chemosphere.2015.09.004

Abstract

To investigate the soil ecological effect of imidacloprid, earthworm Eisenia fetida was exposed to various concentrations of imidacloprid (0.10, 0.50, and 1.00 mg kg(-1) soil) respectively after 7, 14, 21, and 28 d. The effect of imidacloprid on reactive oxygen species (ROS) generation, antioxidant enzymes activity [superoxide dismutase (SOD) and catalase (CAT), glutathione S-transferase enzyme (GST)], malondialdehyde (MDA) content and DNA damage of the E. fetida was investigated. Significant increase of the ROS level was observed. The SOD and GST activity were significantly induced at most exposure intervals. CAT activity was inhibited and reflected a dose-dependent relationship on days 7, 14 and 21. High MDA levels were observed and the olive tail moment (OTM) as well as the percentage of DNA in the comet tail (tail DNA%) in comet assay declined with increasing concentrations and exposure time after 7 d. Our results suggested that the sub-chronic exposure of imidacloprid caused DNA damage and lipid peroxidation (LPO) leading to antioxidant responses in earthworm E. fetida.

Keywords: DNA damage; Eisenia fetida; Imidacloprid; Lipid peroxidation; Oxidative stress.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antioxidants / metabolism
Comet Assay
DNA Damage*
Dose-Response Relationship, Drug
Ecotoxicology
Imidazoles / toxicity*
Lipid Peroxidation / drug effects
Malondialdehyde / metabolism
Neonicotinoids
Nitro Compounds / toxicity*
Oligochaeta / drug effects*
Oligochaeta / enzymology
Oligochaeta / genetics
Oligochaeta / metabolism
Oxidative Stress / drug effects*
Soil Pollutants / toxicity*
Time Factors

Substances

Antioxidants
Imidazoles
Neonicotinoids
Nitro Compounds
Soil Pollutants
imidacloprid
Malondialdehyde