Mechanism study for hypoxia induced differentiation of insulin-producing cells from umbilical cord blood-derived mesenchymal stem cells

Bo Sun; Xian-Hui Meng; Rui Liu; Shancheng Yan; Zhong-Dang Xiao

doi:10.1016/j.bbrc.2015.09.047

Mechanism study for hypoxia induced differentiation of insulin-producing cells from umbilical cord blood-derived mesenchymal stem cells

Biochem Biophys Res Commun. 2015 Oct 23;466(3):444-9. doi: 10.1016/j.bbrc.2015.09.047. Epub 2015 Sep 25.

Authors

Bo Sun¹, Xian-Hui Meng², Rui Liu³, Shancheng Yan⁴, Zhong-Dang Xiao²

Affiliations

¹ State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, PR China; Institute of Microbiology, Seoul National University, Seoul 151-742, South Korea. Electronic address: sunbo@seu.edu.cn.
² State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, PR China.
³ Laboratory of Biophysics, School of Biological Sciences, Seoul National University, Seoul 151-742, South Korea.
⁴ School of Geography and Biological Information, Nanjing University of Posts and Telecommunications, Nanjing 210046, PR China.

PMID: 26392316
DOI: 10.1016/j.bbrc.2015.09.047

Abstract

Recently, we have successfully obtained functional IPCs efficiently from umbilical cord blood-derived mesenchymal stem cells by using hypoxia treatment. In this study, we further elaborated that the improved function and viability of IPCs are the result of the interaction β cell development pathway and c-Met/HGF axis induced by hypoxia. We found that hypoxia induced c-MET elevation is efficiently initiated the early stage differentiation IPCs from MSCs, and HGF improved the fully differentiation of IPCs by inducing the expression of NGN3. This finding may contribute to understanding β cell development and the development of stem cell therapy for diabetes.

Keywords: Differentiation; HGF; Hypoxic; Mesenchymal stem cells; c-Met.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Differentiation
Cell Hypoxia
Fetal Blood / cytology*
Fetal Blood / metabolism*
Humans
Hypoxia-Inducible Factor 1, alpha Subunit / antagonists & inhibitors
Hypoxia-Inducible Factor 1, alpha Subunit / genetics
Hypoxia-Inducible Factor 1, alpha Subunit / metabolism
In Vitro Techniques
Infant, Newborn
Insulin / biosynthesis
Insulin-Secreting Cells / cytology*
Insulin-Secreting Cells / metabolism*
Mesenchymal Stem Cells / cytology*
Mesenchymal Stem Cells / metabolism*
Proto-Oncogene Proteins c-met / antagonists & inhibitors
Proto-Oncogene Proteins c-met / genetics
Proto-Oncogene Proteins c-met / metabolism
RNA Interference

Substances

HIF1A protein, human
Hypoxia-Inducible Factor 1, alpha Subunit
Insulin
Proto-Oncogene Proteins c-met