A novel protein extraction method for identification of mycobacteria using MALDI-ToF MS

La'Tonzia L Adams; Parichat Salee; Kim Dionne; Karen Carroll; Nicole Parrish

doi:10.1016/j.mimet.2015.09.010

A novel protein extraction method for identification of mycobacteria using MALDI-ToF MS

J Microbiol Methods. 2015 Dec:119:1-3. doi: 10.1016/j.mimet.2015.09.010. Epub 2015 Sep 25.

Authors

La'Tonzia L Adams¹, Parichat Salee², Kim Dionne³, Karen Carroll³, Nicole Parrish³

Affiliations

¹ The Johns Hopkins Medical Institutions. Electronic address: latonziaadams@gmail.com.
² The Johns Hopkins Medical Institutions; Chiang Mai University, Chiang Mai, Thailand.
³ The Johns Hopkins Medical Institutions.

PMID: 26392293
DOI: 10.1016/j.mimet.2015.09.010

Abstract

Commercial extraction methods for identification of mycobacteria using MALDI-ToF MS are laborious and time consuming. We have developed a novel extraction method which utilizes a bead beater and zirconia/silica beads to significantly shorten the existing protocol. This novel method provides a more rapid extraction of mycobacteria versus the commercial standard.

Keywords: MALDI-ToF MS; Mycobacterium; Protein extraction.

Publication types

Evaluation Study

MeSH terms

Humans
Mycobacterium / chemistry*
Mycobacterium / genetics
Mycobacterium / growth & development
Mycobacterium / isolation & purification
Mycobacterium Infections / microbiology*
Solid Phase Extraction / instrumentation
Solid Phase Extraction / methods*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods