Detection of N-(1-deoxy-D-fructos-1-yl) Fumonisins B₂ and B₃ in Corn by High-Resolution LC-Orbitrap MS

Toxins (Basel). 2015 Sep 16;7(9):3700-14. doi: 10.3390/toxins7093700.

Abstract

The existence of glucose conjugates of fumonisin B₂ (FB₂) and fumonisin B₃ (FB₃) in corn powder was confirmed for the first time. These "bound-fumonisins" (FB₂ and FB₃ bound to glucose) were identified as N-(1-deoxy-D-fructos-1-yl) fumonisin B₂ (NDfrc-FB₂) and N-(1-deoxy-D-fructos-1-yl) fumonisin B₃ (NDfrc-FB₃) respectively, based on the accurate mass measurements of characteristic ions and fragmentation patterns using high-resolution liquid chromatography-Orbitrap mass spectrometry (LC-Orbitrap MS) analysis. Treatment on NDfrc-FB₂ and NDfrc-FB₃ with the o-phthalaldehyde (OPA) reagent also supported that D-glucose binding to FB₂ and FB₃ molecules occurred to their primary amine residues.

Keywords: Fusarium; LC-Orbitrap MS; N-(1-deoxy-d-fructos-1-yl) fumonisin; corn; fumonisin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, Liquid
  • Food Contamination / analysis
  • Food Microbiology
  • Fumonisins / isolation & purification*
  • Mass Spectrometry
  • Zea mays / microbiology*

Substances

  • Fumonisins
  • fumonisin B2
  • fumonisin B3