Restriction endonucleases from microorganisms isolated in Brazil: an isoschizomer of HaeIII from a thermophilic Bacillus sp

A K Cruz; M Q Pires; V G Lima; C M Morel

Restriction endonucleases from microorganisms isolated in Brazil: an isoschizomer of HaeIII from a thermophilic Bacillus sp

Braz J Med Biol Res. 1989;22(11):1321-8.

Authors

A K Cruz¹, M Q Pires, V G Lima, C M Morel

Affiliation

¹ Departamento de Bioquímica e Biologia Molecular, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, Brasil.

PMID: 2638923

Abstract

1. The isolation and characterization of a restriction endonuclease from a thermophilic strain of Bacillus is described. 2. The enzyme recognizes the palindromic sequence 5'...GGCC...3' as determined by PEI-cellulose chromatography of pancreatic DNAse and snake venom phosphodiesterase digestion products of labelled DNA fragments, analysis of restriction digests and direct sequence analysis. 3. The enzyme, denominated BspBR, is an isoschizomer of HaeIII and BspRI.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacillus / enzymology*
Base Sequence
Brazil
Deoxyribonucleases, Type II Site-Specific / isolation & purification*
Deoxyribonucleases, Type II Site-Specific / metabolism
Electrophoresis, Polyacrylamide Gel

Substances

Deoxyribonucleases, Type II Site-Specific
GGCC-specific type II deoxyribonucleases