Abstract
The filamentous bacteriophage fd, codisplaying antigenic determinants and a single chain antibody fragment directed against the dendritic cell receptor DEC-205, is a promising vaccine candidate for its safety and its ability to elicit innate and adaptive immune response in absence of adjuvants. By using a system vaccinology approach based on RNA-Sequencing (RNA-Seq) analysis, we describe a relevant gene modulation in dendritic cells pulsed with anti-DEC-205 bacteriophages fd. RNA-Seq data analysis indicates that the bacteriophage fd virions are sensed as a pathogen by dendritic cells; they activate the danger receptors that trigger an innate immune response and thus confer a strong adjuvanticity that is needed to obtain a long-lasting adaptive immune response.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptive Immunity*
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Animals
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Antigens, CD / metabolism*
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Bacteriophage M13 / genetics
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Bacteriophage M13 / immunology*
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Bacteriophage M13 / metabolism*
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Cell Surface Display Techniques
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Cluster Analysis
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Dendritic Cells / immunology
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Dendritic Cells / metabolism
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Epitopes, T-Lymphocyte / immunology
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Female
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Gene Expression
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Gene Expression Profiling
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Immunity, Innate*
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Immunomodulation*
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Lectins, C-Type / antagonists & inhibitors
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Lectins, C-Type / metabolism*
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Lymphocyte Activation / immunology
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Mice
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Mice, Transgenic
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Minor Histocompatibility Antigens
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Peptide Library
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Receptors, Cell Surface / antagonists & inhibitors
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Receptors, Cell Surface / metabolism*
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Recombinant Fusion Proteins
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Single-Chain Antibodies / genetics
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Single-Chain Antibodies / metabolism
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T-Lymphocyte Subsets / immunology
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T-Lymphocyte Subsets / metabolism
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Transcriptome
Substances
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Antigens, CD
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DEC-205 receptor
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Epitopes, T-Lymphocyte
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Lectins, C-Type
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Minor Histocompatibility Antigens
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Peptide Library
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Receptors, Cell Surface
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Recombinant Fusion Proteins
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Single-Chain Antibodies