Osteogenic Surface Modification Based on Functionalized Poly-P-Xylylene Coating

Chih-Hao Chang; Shu-Yun Yeh; Bing-Heng Lee; Chia-Jie Chen; Chiao-Tzu Su; Yen-Ting Lin; Chien-Lin Liu; Hsien-Yeh Chen

doi:10.1371/journal.pone.0137017

Osteogenic Surface Modification Based on Functionalized Poly-P-Xylylene Coating

PLoS One. 2015 Sep 17;10(9):e0137017. doi: 10.1371/journal.pone.0137017. eCollection 2015.

Authors

Chih-Hao Chang¹, Shu-Yun Yeh², Bing-Heng Lee¹, Chia-Jie Chen¹, Chiao-Tzu Su², Yen-Ting Lin², Chien-Lin Liu³, Hsien-Yeh Chen²

Affiliations

¹ Department of Orthopedics, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan.
² Department of Chemical Engineering, National Taiwan University, Taipei, Taiwan.
³ Department of Orthopaedics and Traumatology, Taipei Veterans General Hospital, Taipei, Taiwan and School of Medicine, National Yang-Ming University, Taipei, Taiwan.

Abstract

The biotechnology to immobilize biomolecules on material surfaces has been developed vigorously due to its high potentials in medical applications. In this study, a simple and effective method was designed to immobilize biomolecules via amine-N-hydroxysuccinimide (NHS) ester conjugation reaction using functionalized poly-p-xylylene coating on material surfaces. The NHS ester functionalized coating is synthesized via chemical vapor deposition, a facile and solvent-less method, creating a surface which is ready to perform a one-step conjugation reaction. Bone morphogenetic protein 2 (BMP-2) is immobilized onto material surfaces by this coating method, forming an osteogenic environment. The immobilization process is controlled at a low temperature which does not damage proteins. This modified surface induces differentiation of preosteoblast into osteoblast, manifested by alkaline phosphatase (ALP) activity assay, Alizarin Red S (ARS) staining and the expression of osteogenic gene markers, Alpl and Bglap3. With this coating technology, immobilization of growth factors onto material surface can be achieved more simply and more effectively.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

3T3 Cells
Alkaline Phosphatase / biosynthesis
Alkaline Phosphatase / metabolism
Animals
Anthraquinones / metabolism
Bone Morphogenetic Protein 2 / chemistry*
Cell Adhesion / drug effects*
Cell Differentiation / drug effects
Cell Line
Mice
Osteoblasts / cytology*
Osteoblasts / metabolism
Osteogenesis / drug effects*
Osteogenesis / physiology
Polymers / chemistry
Succinimides / chemistry
Surface Properties / drug effects*

Substances

Anthraquinones
Bmp2 protein, mouse
Bone Morphogenetic Protein 2
Polymers
Succinimides
Alizarin Red S
ALPL protein, mouse
Alkaline Phosphatase
N-hydroxysuccinimide

Grants and funding

CHC gratefully acknowledges support from the Ministry of Science and Technology of Taiwan (MOST 103-2633-B-002-014; NSC 102-2320-B-002-002-MY2) and National Taiwan University Hospital-Taipei Veterans General Hospital Joint Research Program (NTUH-TVGH VN103-10). This work is further supported by the Ministry of Science and Technology of Taiwan (NSC 101-2628-E-002-034-MY3; NSC-DAAD 102-2911-I-002-507), and National Taiwan University (102R7745; 103R7745) for HYC. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.