An integrated microfluidic system for bovine DNA purification and digital PCR detection

Qingchang Tian; Ying Mu; Yanan Xu; Qi Song; Bingwen Yu; Congcong Ma; Wei Jin; Qinhan Jin

doi:10.1016/j.ab.2015.08.030

An integrated microfluidic system for bovine DNA purification and digital PCR detection

Anal Biochem. 2015 Dec 15:491:55-7. doi: 10.1016/j.ab.2015.08.030. Epub 2015 Sep 11.

Authors

Qingchang Tian¹, Ying Mu², Yanan Xu³, Qi Song³, Bingwen Yu³, Congcong Ma³, Wei Jin³, Qinhan Jin³

Affiliations

¹ Research Center for Analytical Instrumentation, Institute of CyberSystems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou 310058, Zhejiang, PR China; College of Life Sciences, Zhejiang University, Hangzhou 310058, Zhejiang, PR China.
² Research Center for Analytical Instrumentation, Institute of CyberSystems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou 310058, Zhejiang, PR China. Electronic address: muying@zju.edu.cn.
³ Research Center for Analytical Instrumentation, Institute of CyberSystems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou 310058, Zhejiang, PR China.

PMID: 26364950
DOI: 10.1016/j.ab.2015.08.030

Abstract

In this paper, we described an integrated modularized microfluidic system that contained two distinct functional modules, one for nucleic acids (NA) extraction and the other for digital PCR (dPCR), allowing for detecting the bovine DNA in ovine tissue.

Keywords: DNA purification; Digital PCR; Integrated microfluidic system; Polydimethylsiloxane.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cattle
DNA / analysis*
DNA / isolation & purification
Meat / analysis
Microfluidic Analytical Techniques / instrumentation*
Polymerase Chain Reaction / instrumentation*

Substances

DNA