The insulin degrading enzyme activates ubiquitin and promotes the formation of K48 and K63 diubiquitin

G Grasso; V Lanza; G Malgieri; R Fattorusso; A Pietropaolo; E Rizzarelli; D Milardi

doi:10.1039/c5cc06786c

The insulin degrading enzyme activates ubiquitin and promotes the formation of K48 and K63 diubiquitin

Chem Commun (Camb). 2015 Nov 7;51(86):15724-7. doi: 10.1039/c5cc06786c. Epub 2015 Sep 14.

Authors

G Grasso¹, V Lanza, G Malgieri, R Fattorusso, A Pietropaolo, E Rizzarelli, D Milardi

Affiliation

¹ Dipartimento di Scienze Chimiche, Università degli Studi di Catania, V.le A. Doria 6, 95125 Catania, Italy.

PMID: 26364617
DOI: 10.1039/c5cc06786c

Abstract

We report an ATP-dependent ubiquitin conjugation with IDE which, in turn, promotes Ub-Ub linkages in tube tests. We propose a novel function for IDE as a non-canonical ubiquitin activating enzyme.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenosine Triphosphate / chemistry
Insulysin / chemistry*
Ubiquitin / chemistry*
Ubiquitination
Ubiquitins / chemistry*

Substances

UBD protein, human
Ubiquitin
Ubiquitins
Adenosine Triphosphate
Insulysin