Chd5 Regulates MuERV-L/MERVL Expression in Mouse Embryonic Stem Cells Via H3K27me3 Modification and Histone H3.1/H3.2

Masayasu Hayashi; Kazumitsu Maehara; Akihito Harada; Yuichiro Semba; Kensuke Kudo; Hidehisa Takahashi; Shinya Oki; Chikara Meno; Kenji Ichiyanagi; Koichi Akashi; Yasuyuki Ohkawa

doi:10.1002/jcb.25368

Chd5 Regulates MuERV-L/MERVL Expression in Mouse Embryonic Stem Cells Via H3K27me3 Modification and Histone H3.1/H3.2

J Cell Biochem. 2016 Mar;117(3):780-92. doi: 10.1002/jcb.25368. Epub 2015 Sep 29.

Authors

Masayasu Hayashi^{1

2}, Kazumitsu Maehara¹, Akihito Harada¹, Yuichiro Semba^{1

2}, Kensuke Kudo^{1

3}, Hidehisa Takahashi⁴, Shinya Oki⁵, Chikara Meno⁵, Kenji Ichiyanagi⁶, Koichi Akashi², Yasuyuki Ohkawa¹

Affiliations

¹ Department of Advanced Medical Initiatives, Faculty of Medicine, Kyushu University, Fukuoka 812-8582, Japan.
² Department of Medicine and Biosystemic Science, Faculty of Medicine, Kyushu University, Fukuoka 812-8582, Japan.
³ Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan.
⁴ Department of Biochemistry, Hokkaido University Graduate School of Medicine, Hokkaido 060-8638, Japan.
⁵ Department of Developmental Biology, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan.
⁶ Division of Epigenomics and Development, Medical Institute of Bioregulation, Kyushu University, Fukuoka 812-8582, Japan.

PMID: 26359639
DOI: 10.1002/jcb.25368

Abstract

Chd5 is an essential factor for neuronal differentiation and spermatogenesis and is a known tumor suppressor. H3K27me3 and H3K4un are modifications recognized by Chd5; however, it remains unclear how Chd5 remodels chromatin structure. We completely disrupted the Chd5 locus using the CRISPR-Cas9 system to generate a 52 kbp long deletion and analyzed Chd5 function in mouse embryonic stem cells. Our findings show that Chd5 represses murine endogenous retrovirus-L (MuERV-L/MERVL), an endogenous retrovirus-derived retrotransposon, by regulating H3K27me3 and H3.1/H3.2 function.

Keywords: CHROMATIN REMODELING ENZYME; Chd5; RETROTRANSPOSON; STEM CELL BIOLOGY.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cells, Cultured
Chromatin / metabolism
DNA Helicases / physiology*
Endogenous Retroviruses / genetics
Endogenous Retroviruses / metabolism
Gene Expression
Gene Expression Regulation
Histones / metabolism*
Methylation
Mice
Mouse Embryonic Stem Cells / metabolism*
Protein Processing, Post-Translational*
Proteins / genetics
Proteins / metabolism*
Viral Proteins / genetics
Viral Proteins / metabolism

Substances

Chromatin
Histones
MuERV-L protein, mouse
Proteins
Viral Proteins
DNA Helicases
Chd5 protein, mouse