Using atomic emission spectrometry and EPR analysis metal-ligand homeostasis (MLH) has been studied in epidermal cells of 954 liquidators of the Chernobyl accident and 947 healthy individuals. A possible association of the redox status with the quantitative changes in the MLH, which could be used as discriminators of oxidative/nitrosative stress, attracts special interest. Characteristic features of oxidative stress mainly related to electrogenic metals (Ca, K, Na), were found not only among the liquidators examined, but also in some healthy individuals (18.1%); this suggests the presence of oxidative/nitrosative stress of non-radiation origin. Correlation between intracellular production of nitric oxide (NO) with quantitative changes in the electrogenic metals may indicate the possible involvement of NO in the generation of an electric potential of the cell.
S pomoshch'iu atomno-émissionnoĭ spektrometrii i ÉPR-analiza issledovan metallo-ligandnyĭ gomeostaz (MLG) v kletkakh épidermisa u 954 likvidatorov avarii na Chernobyl'skoĭ AÉS i 947 prakticheski zdorovykh lits. Osobyĭ interes vyzyvaet vozmozhnaia sviaz' redoks-statusa s kolichestvennymi sdvigami v MLG, kotorye mozhno ispol'zovat' v kachestve diskriminatorov okislitel'nogo/nitrozativnogo stressa. Otlichitel'nye priznaki poslednego, kotorye kasalis', glavnym obrazom, élektrogennykh metallov (Ca, K, Na), byli obnaruzheny ne tol'ko sredi likvidatorov avarii, no i u nekotorykh prakticheski zdorovykh lits (18,1%), chto mozhet svidetel'stvovat' o nalichii u nikh okislitel'nogo/nitrozativnogo stressa ne luchevoĭ prirody. Vyiavlennaia sviaz' vnutrikletochnoĭ produktsii oksida azota (NO) s kolichestvennymi sdvigami élektrogennykh metallov mozhet ukazyvat' na vozmozhnoe uchastie NO v generatsii élektricheskogo potentsiala kletki.
Keywords: epidermis; metal-ligand homeostasis; nitric oxide; redox status.