New types of organic-inorganic hybrid nanocomposites based on nanosized Titanium (IV) oxide TiO2 (<100 nm particle size) and carbon nanotubes (CNT, outer diameter 10-15 nm, inner diamentre 2-6 nm, length 0.1-10 µm) and phosphatidilcholine were elaborated for improvement of analytical characteristics of screen printed electrodes. These nanomaterials were employed as an interface for the immobilization of skeletal myoglobin. Electrochemical behavior of myoglobin on such interfaces was characterized with cyclic voltammetry (CV) and square wave voltammetry (SWV). Direct unmediated electron transfer between myoglobin and electrodes modified with organic-inorganic hybrid nanocomposites was registered. TiO2 film and CNT film are biocompartible nanomaterials for myoglobin as was demonstrated with UV-Vis spectra. The midpoint potential of Fe3+/Fe2+ pair of myoglobin corresponded to Е1/2=-0,263 V for CNT film, and Е1/2=-0,468 V for TiO2 nanocomposite (vs. Ag/AgCl reference electrode).
Dlia uluchsheniia analiticheskikh kharakteristik élektrodov, poluchennykh metodom trafaretnoĭ pechati, razrabotany novye tipy nanokompozitnykh organo-neorganicheskikh matrits na osnove nanochastits oksida titana (IV) (TiO2, razmer chastits <100 nm), mnogostenochnykh uglerodnykh nanotrubok (CNT, vneshniĭ diametr 10-15 nm, vnutrenniĭ diametr 2-6 nm, dlina 0,1-10 mkm) i fosfatidilkholina. Issledovany élektroanaliticheskie i élektrokineticheskie kharakteristiki modifitsirovannykh élektrodov s immobilizovannym skeletnym mioglobinom. Affinnye matritsy okharakterizovany metodom tsiklicheskoĭ vol'tamperometrii, a takzhe vol'tamperometricheskogo analiza (kvadratno-volnovoĭ vol'tamperometrii). Pri immobilizatsii mioglobina na modifitsirovannykh élektrodakh nabliudalsia priamoĭ bezmediatornyĭ perenos élektronov mezhdu élektrodami, modifitsirovannymi oksidom titana ili uglerodnymi nanotrubkami, i gemom mioglobina. Okislitel'no-vosstanovitel'nyĭ potentsial (polupotentsial) Fe3+/Fe2+ mioglobina E1/2 = -0,263 V dlia élektrodov, modifitsirovannykh CNT, dlia TiO2 modifikatsii élektrodov E1/2 = -0,468 V (otnositel'no khlorserebrianogo élektroda sravneniia, Ag/AgCl).
Keywords: Titanium oxyde; carbon nanotubes; electron transfer; enzyme electrodes; myoglobin; nanomaterials.