Dynamic microglial modulation of spatial learning and social behavior

Brain Behav Immun. 2016 Jul:55:6-16. doi: 10.1016/j.bbi.2015.09.001. Epub 2015 Sep 5.

Abstract

Microglia are active players in inflammation, but also have important supporting roles in CNS maintenance and function, including modulation of neuronal activity. We previously observed an increase in the frequency of excitatory postsynaptic current in organotypic brain slices after depletion of microglia using clodronate. Here, we describe that local hippocampal depletion of microglia by clodronate alters performance in tests of spatial memory and sociability. Global depletion of microglia by high-dose oral administration of a Csf1R inhibitor transiently altered spatial memory but produced no change in sociability behavior. Microglia depletion and behavior effects were both reversible, consistent with a dynamic role for microglia in the regulation of such behaviors.

Keywords: Clodronate; Csf1 inhibition; Hippocampus; Memory; Microglia depletion.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Behavior, Animal / drug effects*
  • Clodronic Acid / pharmacology*
  • Hippocampus / drug effects*
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Microglia / drug effects*
  • Protein Kinase Inhibitors / pharmacology*
  • Receptor, Macrophage Colony-Stimulating Factor / antagonists & inhibitors*
  • Social Behavior*
  • Spatial Learning / drug effects*

Substances

  • Protein Kinase Inhibitors
  • Clodronic Acid
  • Receptor, Macrophage Colony-Stimulating Factor