Abstract
In the phylogeny of plant polyamine oxidases (PAOs), clade III members from angiosperms, such as Arabidopsis thaliana PAO5 and Oryza sativa PAO1, prefer spermine and thermospermine as substrates and back-convert both of these substrates to spermidine in vitro. A clade III representative of lycophytes, SelPAO5 from Selaginella lepidophylla, also prefers spermine and thermospermine but instead back-converts these substrates to spermidine and norspermidine, respectively. This finding indicates that the clade III PAOs of lycophytes and angiosperms oxidize thermospermine at different carbon positions. We discuss the physiological significance of this difference.
Keywords:
Norspermidine; Polyamine oxidase; Selaginella lepidophylla; Spermidine; Spermine; Thermospermine.
Copyright © 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Chromatography, High Pressure Liquid
-
Dehydration
-
Gene Expression Regulation, Plant / drug effects
-
Molecular Structure
-
Oxidation-Reduction
-
Oxidoreductases Acting on CH-NH Group Donors / classification
-
Oxidoreductases Acting on CH-NH Group Donors / genetics
-
Oxidoreductases Acting on CH-NH Group Donors / metabolism*
-
Phylogeny
-
Plant Proteins / classification
-
Plant Proteins / genetics
-
Plant Proteins / metabolism*
-
Polyamine Oxidase
-
Reverse Transcriptase Polymerase Chain Reaction
-
Selaginellaceae / enzymology*
-
Selaginellaceae / genetics
-
Selaginellaceae / metabolism
-
Spectrophotometry
-
Spermidine / analogs & derivatives*
-
Spermidine / chemistry
-
Spermidine / metabolism
-
Spermine / analogs & derivatives*
-
Spermine / chemistry
-
Spermine / metabolism
-
Tandem Mass Spectrometry
-
Water / metabolism
-
Water / pharmacology
Substances
-
Plant Proteins
-
Water
-
Spermine
-
norspermidine
-
thermospermine
-
Oxidoreductases Acting on CH-NH Group Donors
-
Spermidine