A three-dimensional hierarchical scaffold fabricated by a combined rapid prototyping technique and electrospinning process to expand hematopoietic stem/progenitor cells

Biotechnol Lett. 2016 Jan;38(1):175-81. doi: 10.1007/s10529-015-1952-8. Epub 2015 Sep 7.

Abstract

Objective: To investigate the expansion of hematopoietic stem/progenitor cells (HSPCs) from umbilical cord blood using extracellular matrix (ECM) protein-coated three-dimensional hierarchical scaffolds.

Results: The expansion of HSPCs was evaluated through total nucleated cell (TNC) expansion, immuno-phenotypic analysis, and clonogenic ability. After 7 days of culture, three-dimensional cultures with fibronectin-coated scaffolds achieved the highest fold increase in TNCs (164 ± 6.9 fold) and the highest CD45(+)CD34(+) (35 %) and CD34(+)CD38(-) (32 %) ratios.

Conclusion: Three-dimensional hierarchical scaffolds were coated with ECM protein to simulate a biomimetic environment or niche, and had a significant effect on the expansion potential of HSPCs without changing their phenotype.

Keywords: Electrospinning; Ex vivo expansion; Hematopoietic stem/progenitor cell; Hierarchical scaffold; Rapid prototyping; Stem cell niche.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ADP-ribosyl Cyclase 1 / metabolism
  • Antigens, CD34 / metabolism
  • Biocompatible Materials / chemical synthesis*
  • Cell Culture Techniques / instrumentation
  • Cell Culture Techniques / methods*
  • Cell Proliferation
  • Fibronectins / metabolism*
  • Hematopoietic Stem Cells / cytology*
  • Hematopoietic Stem Cells / immunology
  • Humans
  • Leukocyte Common Antigens / metabolism
  • Stem Cell Niche
  • Surface Properties
  • Umbilical Cord / cytology*

Substances

  • Antigens, CD34
  • Biocompatible Materials
  • Fibronectins
  • Leukocyte Common Antigens
  • PTPRC protein, human
  • ADP-ribosyl Cyclase 1