Isolation and characterization of dextran produced by Leuconostoc citreum NM105 from manchurian sauerkraut

Abstract

A water-soluble exopolysaccharide (EPS) was produced by Leuconostoc ctireum NM105 from homemade manchurian sauerkraut. After culturing the strain in Man-Rogosa-Sharpe medium containing 5% sucrose at 25°C for 48h, the EPS was purified and a yield of 23.5g/L was achieved. The EPS consisted exclusively of glucose and the weight-average molecular weight was 1.01×10(8)Da. The structural characterization of the purified EPS determined by FT-IR, (1)H, (13)C and two-dimensional NMR spectroscopy demonstrated that the glucan contained α-(1→6)-linked d-glucopyranose units, 2,6-linked d-glucopyranose units and terminal α-d-glucopyranose units at a ratio of 1:1:1. The microstructure of the dried dextran appeared a sheet-like smooth glittering and highly branched surface. The NM105 dextran showed high water solubility and excellent water retention. All the results suggested that the highly α-(1→2) branched dextran might have the potential to serve as valuable polymers applied in foods, cosmetics and other fields.

Keywords: Deuterium oxide (PubChem CID: 24602); Dextran; Ethanol (PubChem CID: 702); Exopolysaccharide; Glucose (PubChem CID: 79025); Leuconostoc citreum; Potassium bromide (PubChem CID: 253877); Potassium carbonate (PubChem CID: 11430); Structural characterization; Sucrose (PubChem CID: 5988); Trichloroacetic acid (PubChem CID: 6421); Trifluoroacetic acid (PubChem CID: 6422).