Antimicrobial application of nanofibrous mats self-assembled with quaternized chitosan and soy protein isolate

Yijun Pan; Xingjian Huang; Xiaowen Shi; Yingfei Zhan; Gang Fan; Siyi Pan; Jing Tian; Hongbing Deng; Yumin Du

doi:10.1016/j.carbpol.2015.07.019

Antimicrobial application of nanofibrous mats self-assembled with quaternized chitosan and soy protein isolate

Carbohydr Polym. 2015 Nov 20:133:229-35. doi: 10.1016/j.carbpol.2015.07.019. Epub 2015 Jul 13.

Authors

Yijun Pan¹, Xingjian Huang², Xiaowen Shi³, Yingfei Zhan³, Gang Fan², Siyi Pan², Jing Tian⁴, Hongbing Deng⁵, Yumin Du³

Affiliations

¹ Department of Environmental Science, School of Resource and Environmental Science, Wuhan University, Wuhan 430079, China; College of Food Science and Technology and MOE Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan 430070, China.
² College of Food Science and Technology and MOE Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan 430070, China.
³ Department of Environmental Science, School of Resource and Environmental Science, Wuhan University, Wuhan 430079, China.
⁴ College of Food Science and Technology and MOE Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: jtian.jtian@gmail.com.
⁵ Department of Environmental Science, School of Resource and Environmental Science, Wuhan University, Wuhan 430079, China. Electronic address: hbdeng@whu.edu.cn.

PMID: 26344276
DOI: 10.1016/j.carbpol.2015.07.019

Abstract

Positively charged N-[(2-hydroxy-3-trimethylammonium) propyl] chitosan chloride (HTCC) and negatively charged soy protein isolate (SPI) were alternately assembled on cellulose acetate (CA) electrospun nanofibrous mats via electrostatic layer-by-layer self-assembly technique. CA nanofibrous mats coated with bilayers of HTCC and SPI possessed more orderly-arranged structure than uncoated CA mats according to the observation from scanning electron microscopy images. The average diameter of the nanofibers was enlarged by the increase of the bilayer number. X-ray photoelectron spectroscopy indicated that HTCC and SPI were coated on the surface of the CA mats successfully. The average diameters of inhibition zones of (HTCC/SPI)10.5-films-coated nanofibrous mats against Escherichia coli and Staphylococcus aureus were 9.6mm and 11.53mm, respectively, which demonstrated the highest antimicrobial activity among samples in presented study.

Keywords: Antimicrobial activity; HTCC; Layer-by-layer; Nanofibrous mats; Soy protein isolate.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Infective Agents / chemistry*
Anti-Infective Agents / pharmacology*
Chitosan / chemistry*
Drug Stability
Escherichia coli / drug effects
Nanofibers / chemistry*
Soybean Proteins / chemistry*
Staphylococcus aureus / drug effects
Temperature

Substances

Anti-Infective Agents
Soybean Proteins
Chitosan