Molecular characterization of carbapenem-resistant Pseudomonas aeruginosa strains isolated from patients with urinary tract infections in Southern Poland

Diagn Microbiol Infect Dis. 2015 Nov;83(3):295-7. doi: 10.1016/j.diagmicrobio.2015.07.022. Epub 2015 Jul 29.

Abstract

Due to the clinical threat posed by multidrug-resistant (MDR) Pseudomonas aeruginosa and importance of virulence factors produced in infection, 21 carbapenem-resistant P. aeruginosa were analyzed. 42.8% metallo-beta-lactamases-positive strains were identified. 85.7% of strains were meropenem resistant. 14.2% of strains were MDR; 38%, extensively drug-resistant (XDR). ExoY was present in all strains; exoT, in 95.2%; exoS, in 90.5%; exoU, in 47.6%. Eight XDR strains were typed using multilocus sequence typing: 4 as ST235, 2 as ST260, 2 as ST654 and ST234. MDR P. aeruginosa were isolated from hospitalized patients and among those from the community. Our study demonstrates the serious clinical issues posed by MDR P. aeruginosa and underscores the need for new treatment.

Keywords: Carbapenem-resistance; MLST; Pseudomonas aeruginosa; exo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Aged
  • Aged, 80 and over
  • Anti-Bacterial Agents / pharmacology*
  • Bacterial Proteins / metabolism
  • Carbapenems / pharmacology*
  • Cluster Analysis
  • Female
  • Genes, Bacterial
  • Genetic Variation*
  • Genotype
  • Humans
  • Infant
  • Male
  • Middle Aged
  • Molecular Epidemiology
  • Multilocus Sequence Typing
  • Poland / epidemiology
  • Pseudomonas Infections / epidemiology
  • Pseudomonas Infections / microbiology*
  • Pseudomonas aeruginosa / classification
  • Pseudomonas aeruginosa / drug effects*
  • Pseudomonas aeruginosa / genetics
  • Pseudomonas aeruginosa / isolation & purification
  • Urinary Tract Infections / epidemiology
  • Urinary Tract Infections / microbiology*
  • beta-Lactam Resistance*
  • beta-Lactamases / metabolism

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • Carbapenems
  • beta-Lactamases
  • carbapenemase