The impact of standard chemotherapy on miRNA signature in plasma in AML patients

Leuk Res. 2015 Dec;39(12):1389-95. doi: 10.1016/j.leukres.2015.08.009. Epub 2015 Aug 20.

Abstract

Aim: In our pilot study, we used plasma samples as liquid biopsy to search for miRNA signatures in patients with acute myeloid leukemia (AML) at diagnosis and in remission achieved after standard chemotherapy before planned transplantation.

Material and methods: We examined 10 plasma samples from healthy volunteers and 8 paired samples from patients with AML at diagnosis and in remission using TaqMan MicroRNA Arrays. The results were validated using single-target qPCR reactions run in triplicates.

Results: We selected 6 miRNAs with expressions significantly sensitive to therapy: miR-199b-5p, miR-301b, miR-326, miR-361-5p, miR-625 and miR-655. All selected miRNAs were not or very weakly expressed in healthy individuals. They were abundant in plasma in patients at diagnosis but their levels decreased after chemotherapy.

Conclusion: We detected a therapy sensitive miRNA signature in plasma of patients with AML.

Keywords: Acute myeloid leukemia; Liquid biopsy; Plasma; Remission; Standard chemotherapy; miRNA signature.

Publication types

  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Adult
  • Antineoplastic Combined Chemotherapy Protocols / pharmacology*
  • Antineoplastic Combined Chemotherapy Protocols / therapeutic use
  • Consolidation Chemotherapy
  • Cytarabine / administration & dosage
  • Female
  • Gene Expression Regulation, Leukemic / drug effects*
  • Humans
  • Idarubicin / administration & dosage
  • Leukemia, Myeloid, Acute / blood
  • Leukemia, Myeloid, Acute / drug therapy*
  • Leukemia, Myeloid, Acute / genetics
  • Male
  • MicroRNAs / biosynthesis
  • MicroRNAs / blood*
  • MicroRNAs / genetics
  • Middle Aged
  • Mitoxantrone / administration & dosage
  • Pilot Projects
  • RNA, Neoplasm / biosynthesis
  • RNA, Neoplasm / blood*
  • RNA, Neoplasm / genetics
  • Real-Time Polymerase Chain Reaction
  • Remission Induction
  • Transcriptome*

Substances

  • MIRN301A microRNA, human
  • MIRN326 microRNA, human
  • MIRN361 microRNA, human
  • MIRN625 microRNA, human
  • MIRN655 microRNA, human
  • MicroRNAs
  • RNA, Neoplasm
  • mirn199 microRNA, human
  • Cytarabine
  • Mitoxantrone
  • Idarubicin