Analysis of the Fungicide Boscalid in Horticultural Crops Using an Enzyme-Linked Immunosorbent Assay and an Immunosensor Based on Surface Plasmon Resonance

J Agric Food Chem. 2015 Sep 16;63(36):8075-82. doi: 10.1021/acs.jafc.5b03637. Epub 2015 Sep 4.

Abstract

A direct competitive enzyme-linked immunosorbent assay (dc-ELISA) and an immunosensor based on surface plasmon resonance (SPR-sensor) were developed for fungicide boscalid determination in horticultural crops. To produce antiboscalid monoclonal antibodies (MoAb BSC7 and MoAb BSC72) for these assays, a hapten of boscalid was synthesized and conjugated to keyhole limpet hemocyanin for Balb/c mouse immunization. The working range of the dc-ELISA was 0.8-16 ng/mL with MoAb BSC7 and 2.5-120 ng/mL with MoAb BSC72, and that of the SPR-sensor was 17-80 ng/mL with MoAb BSC7. The dc-ELISA and SPR-sensor were compared for their sensitivity in determining boscalid residues at the maximum residue limit of 1-40 mg/kg for horticultural crops in Japan. Recovery of the spiked boscalid was 85-109% by the SPR-sensor and 100-124% by the dc-ELISA. On real tomato samples, the results obtained by both of these immunoassays correlated well with the results obtained by high-performance liquid chromatography.

Keywords: ELISA; pesticide; surface plasmon resonance.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biphenyl Compounds / analysis*
  • Crops, Agricultural / chemistry*
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Food Contamination / analysis
  • Fungicides, Industrial / analysis*
  • Mice
  • Mice, Inbred BALB C
  • Niacinamide / analogs & derivatives*
  • Niacinamide / analysis
  • Surface Plasmon Resonance / methods*

Substances

  • Biphenyl Compounds
  • Fungicides, Industrial
  • Niacinamide
  • 2-chloro-N-(4-chlorobiphenyl-2-yl)nicotinamide