Variants of human papillomavirus type 16 predispose toward persistent infection

Lei Zhang; Hong Liao; Binlie Yang; Christopher P Geffre; Ai Zhang; Aizhi Zhou; Huimin Cao; Jieru Wang; Zhenbo Zhang; Wenxin Zheng

Variants of human papillomavirus type 16 predispose toward persistent infection

Int J Clin Exp Pathol. 2015 Jul 1;8(7):8453-9. eCollection 2015.

Authors

Lei Zhang¹, Hong Liao², Binlie Yang³, Christopher P Geffre⁴, Ai Zhang¹, Aizhi Zhou¹, Huimin Cao¹, Jieru Wang¹, Zhenbo Zhang⁵, Wenxin Zheng⁶

Affiliations

¹ Department of Obstetrics and Gynecology, The People's Hospital of Shanghai Pudong District Shanghai, China.
² Clinical and Translational Research Center, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine Shanghai, China ; Department of Pathology, University of Arizona College of Medicine Tucson, AZ, USA.
³ Department of Obstetrics and Gynecology, The People's Hospital of Shanghai Pudong District Shanghai, China ; Department of Pathology, University of Arizona College of Medicine Tucson, AZ, USA ; Department of Obstetrics and Gynecology, University of Arizona Tucson, AZ, USA.
⁴ Department of Pathology, University of Arizona College of Medicine Tucson, AZ, USA.
⁵ Department of Pathology, University of Arizona College of Medicine Tucson, AZ, USA ; Department of Obstetrics and Gynecology, Shanghai First People's Hospital, Shanghai Jiaotong University Shanghai, China.
⁶ Department of Pathology, University of Arizona College of Medicine Tucson, AZ, USA ; Department of Obstetrics and Gynecology, University of Arizona Tucson, AZ, USA ; Department of Obstetrics and Gynecology, University of Arizona Tucson, AZ, USA ; Department of Obstetrics and Gynecology, Qilu Hospital, Shandong University Shandong, China ; Arizona Cancer Center, University of Arizona Tucson, AZ, USA.

PMID: 26339417
PMCID: PMC4555745

Abstract

A cohort study of 292 Chinese women was conducted to determine the relationship between human papillomavirus (HPV) type 16 variants and persistent viral infection. Enrolled patients were HPV16 positive and had both normal cytology and histology. Flow-through hybridization and gene chip technology was used to identify the HPV type. A PCR sequencing assay was performed to find HPV16 E2, E6 and E7 gene variants. The associations between these variants and HPV16 persistent infection was analyzed by Fisher's exact test. It was found that the variants T178G, T350G and A442C in the E6 gene, as well as C3158A and G3248A variants in the E2 gene were associated with persistent HPV16 infection. No link was observed between E7 variants and persistent viral infection. Our findings suggest that detection of specific HPV variants would help identify patients who are at high risk for viral persistence and development of cervical neoplasia.

Keywords: Human papillomavirus; persistent infection; variants.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Aged
China
DNA, Viral / genetics*
DNA-Binding Proteins / genetics
Female
Genetic Variation*
Genotype
Human Papillomavirus DNA Tests
Human papillomavirus 16 / genetics*
Human papillomavirus 16 / metabolism
Humans
Middle Aged
Oligonucleotide Array Sequence Analysis
Oncogene Proteins, Viral / genetics
Papillomavirus E7 Proteins / genetics
Papillomavirus Infections / diagnosis
Papillomavirus Infections / virology*
Phenotype
Polymerase Chain Reaction
Repressor Proteins / genetics

Substances

DNA, Viral
DNA-Binding Proteins
E2 protein, Human papillomavirus type 16
E6 protein, Human papillomavirus type 16
Oncogene Proteins, Viral
Papillomavirus E7 Proteins
Repressor Proteins
oncogene protein E7, Human papillomavirus type 16