Draft genome sequence of Streptomyces sp. TP-A0882 reveals putative butyrolactol biosynthetic pathway

Hisayuki Komaki; Natsuko Ichikawa; Akira Hosoyama; Nobuyuki Fujita; Yasuhiro Igarashi

doi:10.1093/femsle/fnv155

Draft genome sequence of Streptomyces sp. TP-A0882 reveals putative butyrolactol biosynthetic pathway

FEMS Microbiol Lett. 2015 Oct;362(20):fnv155. doi: 10.1093/femsle/fnv155. Epub 2015 Sep 3.

Authors

Hisayuki Komaki¹, Natsuko Ichikawa², Akira Hosoyama², Nobuyuki Fujita², Yasuhiro Igarashi³

Affiliations

¹ Biological Resource Center, National Institute of Technology and Evaluation (NBRC), Chiba 292-0818, Japan komaki-hisayuki@nite.go.jp.
² NBRC, Tokyo 151-0066, Japan.
³ Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, Toyama 939-0398, Japan.

PMID: 26337155
DOI: 10.1093/femsle/fnv155

Abstract

We report the draft genome sequence of marine-derived Streptomyces sp. TP-A0882, a producer of buryrolactol, an antifungal γ-lactone-containing polyketide. The genome harbored at least three type I polyketide synthase (PKS), three type II PKS, four nonribosomal peptide synthetase (NRPS) and two hybrid PKS/NRPS gene clusters. Annotation of gene functions enabled us to identify the type I PKS gene cluster for butyrolactol biosynthesis and propose a plausible biosynthetic pathway for this unique polyketide.

Keywords: Actinomycete; antibiotic; biosynthesis; genome mining; thiazostatin.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Proteins / genetics
Bacterial Proteins / metabolism
Base Sequence
Biosynthetic Pathways*
Genome, Bacterial*
Molecular Sequence Data
Polyketides / metabolism*
Streptomyces / genetics*
Streptomyces / metabolism*

Substances

Bacterial Proteins
Polyketides