Comparing Abbott m2000 RealTime HIV test and Roche COBAS Ampliprep/COBAS Taqman HIV test, v2.0 in treated HIV-1 B and non-B subjects with low viraemia

J Med Virol. 2016 Apr;88(4):724-7. doi: 10.1002/jmv.24369. Epub 2015 Sep 21.

Abstract

Viral load testing is a valuable tool in HIV clinical care and research. Discrepancies among diverse viral load assays, especially with regard to non-B HIV-1 subtypes have been reported. Our study aimed to explore the impact of HIV subtype (B versus non-B) on the agreement between CAP/CTM, v2.0 and m2000 RealTime in treated HIV patients, focusing on low viral loads (<200 copies/ml). Our findings indicate that there is a significant difference in the performance of the compared assays in the low-viremic range and non-B subtypes, suggesting that a single assay should be used for follow-up.

Keywords: HIV subtype; agreement study; real time PCR; viral load.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blood / virology*
  • Drug Monitoring / methods*
  • Genotype*
  • HIV Infections / drug therapy*
  • HIV Infections / virology
  • HIV-1 / classification
  • HIV-1 / genetics
  • HIV-1 / isolation & purification*
  • Humans
  • Molecular Diagnostic Techniques / methods*
  • Viral Load / methods*