Identification of candidate biomarker mass (m/z) ranges in serous ovarian adenocarcinoma using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry profiling

Amutha Periyasamy; Gopal Gopisetty; Sridevi Veluswami; Malliga Joyimallaya Subramanium; Rajkumar Thangarajan

doi:10.3109/1354750X.2015.1068862

Identification of candidate biomarker mass (m/z) ranges in serous ovarian adenocarcinoma using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry profiling

Biomarkers. 2015;20(5):292-8. doi: 10.3109/1354750X.2015.1068862.

Authors

Amutha Periyasamy¹, Gopal Gopisetty, Sridevi Veluswami, Malliga Joyimallaya Subramanium, Rajkumar Thangarajan

Affiliation

¹ a Department of Molecular Oncology .

PMID: 26329525
DOI: 10.3109/1354750X.2015.1068862

Abstract

Objective: To differentiate plasma from ovarian cancer and healthy individuals using MALDI-TOF mass spectroscopy.

Materials and methods: MALDI-TOF was used to generate profiles of immuno-depleted plasma samples (89 cancers and 199 healthy individuals) that were fractionated using three types of magnetic beads (HIC8, WCX and IMAC-Cu).

Results: Differentially expressed mass ranges showing >1.5-2-fold change in expression from HIC8 (30), WCX (12) and IMAC-Cu (6) fractions were identified. Cross validation and recognition capability scores for the models indicated discrimination between the classes.

Conclusions: Spectral profiles can differentiate plasma samples of ovarian cancer patients from healthy individuals.

Keywords: HIC8; IMAC-Cu; MALDI-TOF; WCX; ovarian cancer; plasma.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenocarcinoma / blood*
Biomarkers, Tumor / blood*
Blood Proteins / analysis
Electrophoresis, Polyacrylamide Gel
Female
Humans
Molecular Weight
Ovarian Neoplasms / blood*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*

Substances

Biomarkers, Tumor
Blood Proteins