Abstract
Here, we bind the sodium dependent amino acid transporter on nitrilotriacetic acid/polyethylene glycol functionalized gold sensors in detergents and perform a detergent-lipid exchange with phosphatidylcholine. We characterize the LeuT structure in the adsorbed film by magnetic contrast neutron reflection using the predicted model from molecular dynamic simulations.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Transport Systems / chemistry
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Amino Acid Transport Systems / metabolism*
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Detergents / chemistry
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Gold / chemistry
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Lipid Bilayers / chemistry
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Lipid Bilayers / metabolism
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Molecular Dynamics Simulation*
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Nitrilotriacetic Acid / chemistry
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Phosphatidylcholines / chemistry
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Polyethylene Glycols / chemistry
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Quartz Crystal Microbalance Techniques
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Sodium / chemistry
Substances
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Amino Acid Transport Systems
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Detergents
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Lipid Bilayers
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Phosphatidylcholines
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Polyethylene Glycols
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Gold
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Sodium
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Nitrilotriacetic Acid