Single cell nanobiosensors for dynamic gene expression profiling in native tissue microenvironments

Adv Mater. 2015 Oct 21;27(39):6034-8. doi: 10.1002/adma.201502814. Epub 2015 Aug 28.

Abstract

A gold nanorod-locked nucleic acid nano-biosensor for dynamic single-cell gene expression analysis in living cells and tissues is developed. The nanoparticle facilitates endocytic delivery and dynamic monitoring of the gene expression in human umbilical cord endothelial cells, mouse skin tissues, mouse retina tissues, and mouse cornea tissues at the single-cell level.

Keywords: biosensors; locked nucleic acids; nanoparticles; single cells; tissue microenvironments.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Biosensing Techniques*
  • Cells, Cultured
  • Cornea / cytology
  • Cornea / metabolism
  • Cytoplasm / metabolism
  • Endothelial Cells / cytology
  • Endothelial Cells / metabolism
  • Fetal Blood / cytology
  • Fetal Blood / metabolism
  • Gene Expression
  • Gene Expression Profiling / instrumentation*
  • Gene Expression Profiling / methods
  • Gold* / chemistry
  • Humans
  • Metal Nanoparticles* / chemistry
  • Mice, Inbred C57BL
  • Nanotubes* / chemistry
  • Nucleic Acid Probes / chemistry
  • Particle Size
  • RNA, Messenger / metabolism
  • Retina / cytology
  • Retina / metabolism
  • Single-Cell Analysis / instrumentation
  • Single-Cell Analysis / methods
  • Stem Cells / cytology
  • Stem Cells / metabolism

Substances

  • Nucleic Acid Probes
  • RNA, Messenger
  • Gold