Changes in Endosymbiotic Bacteria of Brown Planthoppers During the Process of Adaptation to Different Resistant Rice Varieties

Abstract

The specific primers of five species of endosymbiotic bacteria were designed to determine their numbers in three virulent populations of brown planthopper, Nilapavata lugens Stål, and to assess changes during adaptation to different resistant varieties using fluorescent quantitative PCR. The results showed that Chryseobacterium was the dominant bacteria in all three populations of brown planthopper, followed by Acinetobacter in TN1 population, Arsenophonus and Serratia in Mudgo population, and Arthrobacter and Acinetobacter in ASD7 population. When the TN1 population of brown planthopper was transferred to ASD7 (with resistant gene bph2) rice plants, Chryseobacterium was still the dominant bacteria, but the originally subdominant Acinetobacter declined to a level that was not significantly different from that of other endosymbiotic bacteria. After they were transferred to Mudgo (with resistant gene Bph1), Serratia and Arsenophonus increased significantly and became the dominant bacteria. However, they declined to a level that was not significantly different from that of the three other species after two generations. When ASD7 and Mudgo populations of brown planthopper were transferred to the susceptible variety TN1, the community of endosymbiotic bacteria in the ASD7 population of brown planthopper showed no significant changes. However, the numbers of Acinetobacter and Arthrobacter in the Mudgo population of brown planthopper exhibited a transient increase and returned to their original levels after two generations. After the Mudgo population of brown planthopper was transferred to ASD7 rice plants, the quantity of endosymbiotic bacteria fluctuated, but the bacterial structure did not change significantly. However, after the ASD7 population of brown planthopper was transferred to the Mudgo rice plants, the bacterial structure changed significantly. Serratia and Arsenophonus increased significantly and became dominant. Although Serratia and Arsenophonus decreased significantly after a generation, they were still greater than Chryseobacterium. It was presumed that Chryseobacterium was dominant in all three populations of virulent brown planthoppers, but had no significant effect on virulence variation of brown planthopper. However, Serratia and Arsenophonus might be correlated with virulence variation of brown planthopper.

Keywords: Nilaparvata lugens; brown planthopper; endosymbiotic bacteria; resistant rice variety; virulence.