MiR-378b Promotes Differentiation of Keratinocytes through NKX3.1

Xi-Liang Wang; Tao Zhang; Jing Wang; Dian-Bao Zhang; Feng Zhao; Xue-Wen Lin; Zhe Wang; Ping Shi; Xi-Ning Pang

doi:10.1371/journal.pone.0136049

MiR-378b Promotes Differentiation of Keratinocytes through NKX3.1

PLoS One. 2015 Aug 27;10(8):e0136049. doi: 10.1371/journal.pone.0136049. eCollection 2015.

Authors

Xi-Liang Wang¹, Tao Zhang¹, Jing Wang², Dian-Bao Zhang¹, Feng Zhao¹, Xue-Wen Lin¹, Zhe Wang³, Ping Shi⁴, Xi-Ning Pang¹

Affiliations

¹ Key Laboratory of Cell Biology, Ministry of Public Health of China, Department of Stem Cells and Regenerative Medicine, China Medical University, Shenyang, 110122, China.
² Department of Anus and Intestine Surgery, the First Affiliated Hospital, China Medical University, Shenyang, 110001, China.
³ Department of Blood Transfusion, Shengjing Hospital of China Medical University, Shenyang, 110004, China.
⁴ Department of General Practice, the First Affiliated Hospital, China Medical University, Shenyang, 110001, China.

Abstract

MicroRNA (miRNA) is a kind of short non-coding RNA, involved in various cellular processes. During keratinocyte differentiation, miRNAs act as important regulators. In this study, we demonstrated by microarray assay that the expression of miR-378b significantly increased during keratinocytes differentiation. Our findings showed that miR-378b could inhibit proliferation, migration and differentiation in keratinocytes. Luciferase reporter assays showed that miR-378b directly target NKX3.1. Silencing of NKX3.1 could coincide with the effects of miR-24 overexpression. In conclusion, our results demonstrate miR-378b promote keratinocytes differentiation by targeting NKX3.1. Manipulation of miR-378b may afford a new strategy to clinic treatment of skin injury and repair.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Blotting, Western
Cell Differentiation*
Cell Movement
Cell Proliferation
Cells, Cultured
Homeodomain Proteins / genetics
Homeodomain Proteins / metabolism*
Humans
Keratinocytes / cytology*
Keratinocytes / metabolism
MicroRNAs / genetics*
RNA, Messenger / genetics
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
Skin / cytology*
Skin / metabolism
Transcription Factors / genetics
Transcription Factors / metabolism*

Substances

Homeodomain Proteins
MIRN378 microRNA, human
MicroRNAs
NKX3-1 protein, human
RNA, Messenger
Transcription Factors

Grants and funding

This study was supported by the grants from the National Basic Research Program of China (http://www.most.gov.cn) Grants 2012CB518103 (to XP), the National Natural Science Foundation of China (http://www.nsfc.gov.cn/) Grants 81450017 (to XP), the Science and Technology Department of Liaoning Province (http://www.lninfo.gov.cn/) Grants 2012225080 (to XP) and Grants 2014305012 (to JW), the Science and Technology Bureau of Shenyang City (http://www.syskjj.gov.cn/websiteManageMain_init.do) Grants F11-262-9-01 (to PS) and Grants F15-157-1-00 (to XP). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.