AFM single-cell force spectroscopy links altered nuclear and cytoskeletal mechanics to defective cell adhesion in cardiac myocytes with a nuclear lamin mutation

Nucleus. 2015;6(5):394-407. doi: 10.1080/19491034.2015.1084453.

Abstract

Previous investigations suggested that lamin A/C gene (LMNA) mutations, which cause a variety of human diseases including muscular dystrophies and cardiomyopathies, alter the nuclear mechanical properties. We hypothesized that biomechanical changes may extend beyond the nucleus.

Keywords: AFM; Young Modulus; cardiomyocytes; cardiomyopathy; cell physiology; lamin A/C; relaxation force test.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Newborn
  • Biomechanical Phenomena
  • Cell Adhesion
  • Cell Nucleus / metabolism*
  • Cell Shape
  • Cytoskeleton / metabolism*
  • Elastic Modulus
  • Humans
  • Lamin Type A / genetics*
  • Microscopy, Atomic Force / methods*
  • Models, Biological
  • Mutant Proteins / metabolism
  • Mutation / genetics*
  • Myocytes, Cardiac / cytology*
  • Myocytes, Cardiac / metabolism*
  • Rats, Wistar
  • Spectrum Analysis / methods*
  • Tubulin / metabolism
  • Viscosity

Substances

  • Lamin Type A
  • Mutant Proteins
  • Tubulin