Microtus ochrogaster, a small vole, found in central North America. Recently the genome sequencing had been done, but no more information of its mitochondrial reported. Herein, we first assembled the complete mitochondrial genome sequence of Microtus ochrogaster. It is a 16 292 bp long sequence with most mitogenome's characteristic structure, 13 protein-coding genes, two of rRNA genes, 22 of tRNA genes, one D-loop region, one repeat region, and three STS regions. The GC-content of our fresh sequence is 40%. It can verify the accuracy and utility of new determined mitogenome sequences by the phylogenetic analysis, based on the whole mitogenome alignment with Microtus fortis calamorum, Microtus levis, and Microtus rossiaemeridionalis, which is closest relative to Microtus ochrogaster. We expect that using the full mitogenome to address taxonomic issues and study the related evolution events.
Keywords: Microtus ochrogaster; mitogenome; phylogenetic.