Integrated utilization of red radish seeds for the efficient production of seed oil and sulforaphene

Food Chem. 2016 Feb 1:192:541-7. doi: 10.1016/j.foodchem.2015.07.051. Epub 2015 Jul 11.

Abstract

Supercritical CO2 was used to obtain seed oil from red radish seeds. The influence of pressure, temperature, CO2 flow rate and time on extraction yield of oil were investigated in detail. The maximum extraction yield of oil was 92.07 ± 0.76% at the optimal extraction conditions. The physicochemical properties and fatty acid composition of oil indicated that the seed oil can be used as a dietary oil. Meanwhile, the high purity sulforaphene (96.84 ± 0.17%) was separated by solvent extraction coupled with preparative high performance liquid chromatography from red radish seed meal. The initial pH, R, extraction temperature and extraction time for each cycle had a considerable influence both on the extraction yield and purity of sulforaphene of crude product. The extraction of oil was directly responsible for an increase of 18.32% in the yield of sulforaphene.

Keywords: High performance liquid chromatography; Red radish; Seed oil; Sulforaphene; Supercritical carbon dioxide.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chemical Phenomena
  • Chromatography, High Pressure Liquid / methods
  • Chromatography, Supercritical Fluid / methods
  • Dietary Fats, Unsaturated
  • Fatty Acids / analysis
  • Isothiocyanates / isolation & purification*
  • Plant Oils / chemistry
  • Plant Oils / isolation & purification*
  • Pressure
  • Raphanus / chemistry*
  • Seeds / chemistry*
  • Temperature

Substances

  • Dietary Fats, Unsaturated
  • Fatty Acids
  • Isothiocyanates
  • Plant Oils
  • sulphoraphene