First molecular identification of Babesia gibsoni in dogs from Slovakia, central Europe

Ticks Tick Borne Dis. 2016 Feb;7(1):54-59. doi: 10.1016/j.ttbdis.2015.08.004. Epub 2015 Aug 10.

Abstract

Canine babesiosis is a severe and potentially life threatening infection. In Europe, Babesia canis is considered to be the most common species responsible for the disease. We report two cases of babesiosis caused by Babesia gibsoni. The polymerase chain reaction, restriction fragment length polymorphism analysis and further sequencing of 18S rRNA gene fragments from blood samples of both dogs revealed the identity of isolates with B. gibsoni genotypes from other dogs worldwide. This species was previously not known to infect dogs in Slovakia. It is resistant to traditional anti-babesial therapy. Therefore, correct diagnosis is crucial for the successful treatment, especially in dogs with hemolytic anemia and febrile conditions.

Keywords: B. gibsoni; PCR; Pit bull terrier; RFLP; Slovakia.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Bacterial Agents / administration & dosage
  • Anti-Bacterial Agents / therapeutic use
  • Antimalarials / administration & dosage
  • Antimalarials / therapeutic use
  • Atovaquone / administration & dosage
  • Atovaquone / therapeutic use
  • Azithromycin / administration & dosage
  • Azithromycin / therapeutic use
  • Babesia / classification
  • Babesia / isolation & purification*
  • Babesiosis / drug therapy
  • Babesiosis / epidemiology
  • Babesiosis / parasitology*
  • Dog Diseases / drug therapy
  • Dog Diseases / epidemiology
  • Dog Diseases / parasitology*
  • Dogs
  • Drug Combinations
  • Genotype
  • Male
  • Proguanil / administration & dosage
  • Proguanil / therapeutic use
  • Slovakia / epidemiology

Substances

  • Anti-Bacterial Agents
  • Antimalarials
  • Drug Combinations
  • atovaquone, proguanil drug combination
  • Azithromycin
  • Proguanil
  • Atovaquone