Detection of DNA double-strand breaks in boron neutron capture reaction

Emiko Okamoto; Tetsuya Yamamoto; Kei Nakai; Fumiyo Yoshida; Akira Matsumura

doi:10.1016/j.apradiso.2015.08.019

Detection of DNA double-strand breaks in boron neutron capture reaction

Appl Radiat Isot. 2015 Dec:106:185-8. doi: 10.1016/j.apradiso.2015.08.019. Epub 2015 Aug 17.

Authors

Emiko Okamoto¹, Tetsuya Yamamoto², Kei Nakai³, Fumiyo Yoshida², Akira Matsumura²

Affiliations

¹ Department of Neurosurgery, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan; Doctoral Program in Clinical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan.
² Department of Neurosurgery, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan.
³ Department of Neurosurgery, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan. Electronic address: knakai@md.tsukuba.ac.jp.

PMID: 26302660
DOI: 10.1016/j.apradiso.2015.08.019

Abstract

We evaluated DNA double-strand breaks (DSBs) induced by boron neutron capture reaction (BNCR) using plasmid DNA, boron solution, and gel electrophoresis. The amount of the linear form of DNA produced by DSBs increased with the neutron-beam irradiation dose. The amount of the open-circular form of DNA produced by single-strand breaks (SSBs) increased with the neutron-beam irradiation dose and the (10)B concentration. The model facilitated quantification of BNCR-induced DSBs and SSBs, irrespective of the DNA repair mechanism.

Keywords: DNA damage; Double-strand break; Neutron capture therapy.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Boron Neutron Capture Therapy*
DNA Damage*
Electrophoresis, Agar Gel