In situ high-valued utilization and transformation of sugars from Dioscorea zingiberensis C.H. Wright for clean production of diosgenin

Yun Bai; Liwei Zhang; Wenwen Jin; Mi Wei; Pengpeng Zhou; Guihua Zheng; Lili Niu; Lin Nie; Yongliang Zhang; Haiyan Wang; Longjiang Yu

doi:10.1016/j.biortech.2015.08.010

In situ high-valued utilization and transformation of sugars from Dioscorea zingiberensis C.H. Wright for clean production of diosgenin

Bioresour Technol. 2015 Nov:196:642-7. doi: 10.1016/j.biortech.2015.08.010. Epub 2015 Aug 8.

Authors

Yun Bai¹, Liwei Zhang¹, Wenwen Jin¹, Mi Wei², Pengpeng Zhou¹, Guihua Zheng¹, Lili Niu³, Lin Nie¹, Yongliang Zhang¹, Haiyan Wang¹, Longjiang Yu⁴

Affiliations

¹ Institute of Resource Biology and Biotechnology, Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China; Key Laboratory of Molecular Biophysics Ministry of Education, Huazhong University of Science and Technology, Wuhan 430074, China.
² Key Laboratory for Quality Control of Characteristic Fruits and Vegetables of Hubei Province, College of Life Science and Technology, Hubei Engineering University, Xiaogan 432000, China.
³ Institute of Resource Biology and Biotechnology, Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.
⁴ Institute of Resource Biology and Biotechnology, Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China; Key Laboratory of Molecular Biophysics Ministry of Education, Huazhong University of Science and Technology, Wuhan 430074, China; Wuhan Institute of Biotechnology, Wuhan 430075, China. Electronic address: yulongjiang@hust.edu.cn.

PMID: 26299979
DOI: 10.1016/j.biortech.2015.08.010

Abstract

The industrial production of diosgenin in China generates a large amount of high-sugar wastes with low bioavailability, which causes serious pollution to the environment. In this study, a new clean and efficient process for the production of diosgenin was developed using sugars through in situ high-valued transformation. The sugar mixture from Dioscorea zingiberensis C.H. Wright contained abundant beneficial components. Nine typical microorganisms that produced intracellular products were evaluated. Saccharopolyspora spinosa was selected for recursive protoplast fusion to increase the spinosad yield by 46.3% compared with that of the wildtype. Diosgenin and spinosad co-production was conducted in a 100L bioreactor, with pH controlled by adding glucose. The biological oxygen demand of the effluent water decreased from 15,000mg/L to 450mg/L; hence, the proposed process is environment friendly.

Keywords: Co-production; Diosgenin; High-valued utilization; In situ; Spinosad.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bioreactors*
Carbohydrate Metabolism
China
Dioscorea* / chemistry
Dioscorea* / metabolism
Diosgenin* / analysis
Diosgenin* / metabolism

Substances

Diosgenin