Antigen-Antibody Affinity for Dry Eye Biomarkers by Label Free Biosensing. Comparison with the ELISA Technique

Sensors (Basel). 2015 Aug 13;15(8):19819-29. doi: 10.3390/s150819819.

Abstract

The specificity and affinity of antibody-antigen interactions is a fundamental way to achieve reliable biosensing responses. Different proteins involved with dry eye dysfunction: ANXA1, ANXA11, CST4, PRDX5, PLAA and S100A6; were validated as biomarkers. In this work several antibodies were tested for ANXA1, ANXA11 and PRDX5 to select the best candidates for each biomarker. The results were obtained by using Biophotonic Sensing Cells (BICELLs) as an efficient methodology for label-free biosensing and compared with the Enzyme-Linked Immuno Sorbent Assay (ELISA) technique.

Keywords: biosensors; dry eye biomarkers; label-free immunoassay; photonic sensing cells BICELLs.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Annexins / immunology
  • Antibodies, Monoclonal / metabolism
  • Antibody Affinity / immunology*
  • Antigens / metabolism*
  • Biomarkers / analysis*
  • Biosensing Techniques / methods*
  • Calibration
  • Dry Eye Syndromes / diagnosis*
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Female
  • Kinetics
  • Mice, Inbred BALB C
  • Optical Phenomena
  • Staining and Labeling

Substances

  • Annexins
  • Antibodies, Monoclonal
  • Antigens
  • Biomarkers