Comparative gene-expression profiling of CD133(+) and CD133(-) D10 melanoma cells

Future Oncol. 2015;11(17):2383-93. doi: 10.2217/fon.15.174. Epub 2015 Aug 19.

Abstract

Aims: The present study aimed to compare the gene-expression profiling of CD133(+) and CD133(-) D10 cells.

Materials & methods: Cancer stem cell-like properties and gene-expression profiling of CD133(+) D10 cells versus CD133(-) cells were evaluated.

Results: The CD133(+) D10 cells showed significantly higher clonogenic and spheroid forming potential, also higher expression of stemness genes NANOG and OCT4A compared with the CD133(-) cells. Gene-expression profiling of CD133(+) versus CD133(-) D10 cells revealed that 130 genes including ABC transporter superfamily (ABCC1, ABCG2 and ABCC6) were upregulated, while 61 genes including apoptosis modifying genes (CASP8 and TNFRSF4) were downregulated.

Conclusion: We conclude that many genes involved in drug resistance and tumor aggressiveness are upregulated in CD133(+) D10 cells and targeting them might be an efficient strategy for treatment of melanoma.

Keywords: CD133; D10 cell line; cDNA microarray; cancer stem cells; melanoma.

MeSH terms

  • AC133 Antigen
  • Antigens, CD / genetics*
  • Antigens, CD / metabolism*
  • Biomarkers, Tumor
  • Cell Line, Tumor
  • Computational Biology / methods
  • Gene Expression Profiling*
  • Gene Expression Regulation, Neoplastic*
  • Glycoproteins / genetics*
  • Glycoproteins / metabolism*
  • Humans
  • Immunophenotyping
  • Melanoma / genetics*
  • Melanoma / metabolism*
  • Peptides / genetics*
  • Peptides / metabolism*
  • Protein Interaction Mapping
  • Protein Interaction Maps
  • Transcriptome*

Substances

  • AC133 Antigen
  • Antigens, CD
  • Biomarkers, Tumor
  • Glycoproteins
  • PROM1 protein, human
  • Peptides