Low Expression of miR-491 Promotes Esophageal Cancer Cell Invasion by Targeting TPX2

Cell Physiol Biochem. 2015;36(6):2263-73. doi: 10.1159/000430190. Epub 2015 Jul 24.

Abstract

Background/aims: MicroRNAs (miRNAs) have a key role in carcinogenesis and cancer development, but the role of miRNAs in the progression of esophageal cancer (EC) remains unclear.

Methods: The TE-1 and Eca-109 EC cell lines were used. The expression of miR-491 and candidate gene TPX2 in EC samples (n=99) were detected by RT-PCR. The cells invasive ability was determined by transwell assay. The luciferase reporter assay was used to confirm the regulation mechanism.

Results: A decreased expression of miR-491 was detected in the EC clinical samples compared with the corresponding adjacent tumor tissues. Aberrant expression of miR-491 regulated cells invasion and EMT markers. Furthermore, we verified that TPX2 was a target gene of miR-491.

Conclusions: miR-491 may play a critical role in EC.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Base Sequence
  • Cell Cycle Proteins / genetics*
  • Cell Cycle Proteins / metabolism
  • Cell Line, Tumor
  • Cell Proliferation
  • Down-Regulation / genetics
  • Epithelial-Mesenchymal Transition / genetics
  • Esophageal Neoplasms / genetics*
  • Esophageal Neoplasms / pathology*
  • Female
  • Gene Expression Regulation, Neoplastic*
  • Gene Knockdown Techniques
  • Humans
  • Male
  • MicroRNAs / genetics
  • MicroRNAs / metabolism
  • Microtubule-Associated Proteins / genetics*
  • Microtubule-Associated Proteins / metabolism
  • Middle Aged
  • Molecular Sequence Data
  • Neoplasm Invasiveness
  • Nuclear Proteins / genetics*
  • Nuclear Proteins / metabolism
  • RNA, Small Interfering / metabolism

Substances

  • Cell Cycle Proteins
  • MIRN491 microRNA, human
  • MicroRNAs
  • Microtubule-Associated Proteins
  • Nuclear Proteins
  • RNA, Small Interfering
  • TPX2 protein, human