Antimicrobial activity and synergism of Sami-Hyanglyun-Hwan with ciprofloxacin against methicillin-resistant Staphylococcus aureus

Jang-Gi Choi; Ji-Young Choi; Su-Hyun Mun; Ok-Hwa Kang; Preeti Bharaj; Dong-Won Shin; Myong-Soo Chong; Dong-Yeul Kwon

doi:10.1016/j.apjtm.2015.06.010

Antimicrobial activity and synergism of Sami-Hyanglyun-Hwan with ciprofloxacin against methicillin-resistant Staphylococcus aureus

Asian Pac J Trop Med. 2015 Jul;8(7):538-42. doi: 10.1016/j.apjtm.2015.06.010. Epub 2015 Jul 9.

Authors

Jang-Gi Choi¹, Ji-Young Choi², Su-Hyun Mun³, Ok-Hwa Kang⁴, Preeti Bharaj⁵, Dong-Won Shin⁶, Myong-Soo Chong², Dong-Yeul Kwon⁷

Affiliations

¹ Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University, Wonkwang Oriental Medicines Research Institute, Jeonbuk 570-749, Republic of Korea; Center of Excellence in Infectious Disease Research, Department of Biomedical Sciences, Paul L. Foster School of Medicine, Texas Tech University Health Sciences Center, ElPaso, TX 79905, USA.
² Department of Third Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk 570-749, Republic of Korea.
³ BK21 Plus Team, Professional Graduate School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk 570-749, Republic of Korea.
⁴ Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University, Wonkwang Oriental Medicines Research Institute, Jeonbuk 570-749, Republic of Korea.
⁵ Center of Excellence in Infectious Disease Research, Department of Biomedical Sciences, Paul L. Foster School of Medicine, Texas Tech University Health Sciences Center, ElPaso, TX 79905, USA.
⁶ Department of Oriental Medicine Resources, Sunchon National University, Jeonnam 540-742, Republic of Korea.
⁷ Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University, Wonkwang Oriental Medicines Research Institute, Jeonbuk 570-749, Republic of Korea. Electronic address: sssimi@wku.ac.kr.

PMID: 26276284
DOI: 10.1016/j.apjtm.2015.06.010

Abstract

Objective: To investigate the antibacterial activity of SHH extracted with either water or ethanol against methicillin-resistant Staphylococcus aureus (MRSA) and combinatory antimicrobial effect with ciprofloxacin (CIP) by time kill assay and checkerboard dilution test.

Methods: The antibacterial activity determined by broth dilution method indicated that the antibacterial activity of Sami-Hyanglyun-Hwan (SHH) water extract (SHHW) and SHH ethanol extract (SHHE) ranged from 250 to 2000 μg/mL and 125 to 1000 μg/mL against MRSA, respectively.

Results: In the checkerboard method, the combinations of SHHE with CIP had a partial synergistic or synergistic effect against MRSA. The time-kill curves showed that a combined SHHE and CIP treatment reduced the bacterial counts dramatically after 24 h.

Conclusions: The present study demonstrates the therapeutic ability of SHHE against MRSA infections.

Keywords: Ciprofloxacin; Methicillin-resistant Staphylococcus aureus; Sami-Hyanglyun-Hwan; Synergy.